type a strain atcc 3624 (ATCC)

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ATCC type a strain atcc 3624
Type A Strain Atcc 3624, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+atcc+3624/Clostridium+perfringens+(Veillon+and+Zuber)+Hauduroy+et+al/pmc11364075-62-16-19
Average 95 stars, based on 155 article reviews

type a strain atcc 3624 - by Bioz Stars, 2026-09

95/100 stars

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Comparison:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: For all three ATCC 3624-related strains, the 16S RNA expression levels were similar. .. Comparison of growth rates and toxin production by wild-type ATCC 3624 versus an ATCC 3624 agrB-null mutant or complementing strain in TY medium. .. When growth curve analyses were performed (Fig. 2A), wild-type ATCC 3624, its agrB-null mutant, and the complementing strain all grew similarly in TY medium at 37°C.

Mutagenesis:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: For all three ATCC 3624-related strains, the 16S RNA expression levels were similar. .. Comparison of growth rates and toxin production by wild-type ATCC 3624 versus an ATCC 3624 agrB-null mutant or complementing strain in TY medium. .. When growth curve analyses were performed (Fig. 2A), wild-type ATCC 3624, its agrB-null mutant, and the complementing strain all grew similarly in TY medium at 37°C.

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: The present study next tested the pathogenicity of C. perfringens type A strain ATCC 3624 and its derivatives in a mouse model of clostridial myonecrosis. .. For this analysis, 106 CFU of wild-type ATCC 3624, the agrB-null mutant and complementing strain were each inoculated intramuscularly into the left hind leg of eight male or female BALB/c mice (weighing 20 to 25 g) per group. ..

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: In the present study, C. perfringens type A strain ATCC 3624 and an isogenic agrB-null mutant were tested in a mouse model of gas gangrene. .. When mice were intramuscularly challenged with 106 CFU of wild-type ATCC 3624, severe myonecrosis and leukocyte aggregation occurred by 4 h. Similar numbers of an agrB-null mutant strain produced significantly less severe changes in the skeletal muscle of challenged mice. ..

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: .. Association May/June 2020 Volume 5 Issue 3 e00500-20 msphere.asm.org 5 of C. perfringens with the microscopic lesions in mice inoculated with wild-type ATCC 3624, the agrB mutant (Fig. 3C), or complementing strain was confirmed by IHC using an indirect immunoperoxidase technique. ..

Polymerase Chain Reaction:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: The present study constructed an agrB-null mutant of type A strain ATCC 3624 in order to begin evaluating whether the Agr-like quorumsensing system regulates the ability of type A strains to produce toxins and cause gas gangrene in a mouse model. For this purpose, the Clostridium-modified TargeTronmediated insertional mutagenesis method (32) was used to construct an ATCC 3624 agrB-null mutant, as demonstrated by PCR using primers that flank the intron insertion site and are specific for internal agrB open reading frame (ORF) sequences (Fig. 1A). .. With template DNA from wild-type ATCC 3624, these internal PCR primers specifically amplified a PCR product of 536 bp. ..

Amplification:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: The present study constructed an agrB-null mutant of type A strain ATCC 3624 in order to begin evaluating whether the Agr-like quorumsensing system regulates the ability of type A strains to produce toxins and cause gas gangrene in a mouse model. For this purpose, the Clostridium-modified TargeTronmediated insertional mutagenesis method (32) was used to construct an ATCC 3624 agrB-null mutant, as demonstrated by PCR using primers that flank the intron insertion site and are specific for internal agrB open reading frame (ORF) sequences (Fig. 1A). .. With template DNA from wild-type ATCC 3624, these internal PCR primers specifically amplified a PCR product of 536 bp. ..

Western Blot:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: Therefore, the present study evaluated whether the 6-R peptide can also inhibit CPA or PFO production by type A strain ATCC 3624. .. For this analysis, three different concentrations of the 6-R peptide were added to TY broth inoculated with wild-type ATCC 3624, followed by CPA or PFO Western blot analysis of CPA or PFO levels in 5-h culture supernatants of those cultures. ..

Produced:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: In the present study, C. perfringens type A strain ATCC 3624 and an isogenic agrB-null mutant were tested in a mouse model of gas gangrene. .. When mice were intramuscularly challenged with 106 CFU of wild-type ATCC 3624, severe myonecrosis and leukocyte aggregation occurred by 4 h. Similar numbers of an agrB-null mutant strain produced significantly less severe changes in the skeletal muscle of challenged mice. ..

Injection:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: It has been hypothesized that the coordinated production of extracellular virulence factors when a FIG 6 Inhibitory effects of the 6-R peptide on the virulence of ATCC 3624 in a mouse model of C. perfringens type A-induced gas gangrene. .. The hind limbs of mice were injected with 106 CFU of wild-type ATCC 3624 with or without the 6-R peptide (100 M) and incubated for 4 h. (A) Macroscopic pathology of mice injected with the corresponding treatment. (B) Microscopic pathology of mice injected with the corresponding treatment. .. Note the severe myonecrosis in a mouse receiving the ATCC 3624 strain only.

Incubation:

Article Title: The Agr-Like Quorum-Sensing System Is Important for Clostridium perfringens Type A Strain ATCC 3624 To Cause Gas Gangrene in a Mouse Model
Article Snippet: It has been hypothesized that the coordinated production of extracellular virulence factors when a FIG 6 Inhibitory effects of the 6-R peptide on the virulence of ATCC 3624 in a mouse model of C. perfringens type A-induced gas gangrene. .. The hind limbs of mice were injected with 106 CFU of wild-type ATCC 3624 with or without the 6-R peptide (100 M) and incubated for 4 h. (A) Macroscopic pathology of mice injected with the corresponding treatment. (B) Microscopic pathology of mice injected with the corresponding treatment. .. Note the severe myonecrosis in a mouse receiving the ATCC 3624 strain only.

Immunohistochemistry: